Yes, if your partner genuinely works in the business and the pay is reasonable for the work they do. HMRC treats family wages like any other business expense: it has to be a real cost of the business, in line with what you would pay an unrelated person for the same work.
How you do it depends on your structure:
If the pay is more than the work justifies, HMRC can refuse the deduction while your partner is still taxed on what they received. Keep a record of their duties and hours, check minimum wage rules apply, and pay them through payroll rather than from your personal account.
Legislation and guidance: Income Tax (Trading and Other Income) Act 2005, section 34 (wholly and exclusively); Corporation Tax Act 2009, section 54 (wholly and exclusively); HMRC Enquiry Manual EM3911 (family wages).
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